Al-Jumaa, Z., AL-Taee, S., Jaber, M., Rahhawi, A. (2024). Histopathological Alteration and Molecular Detection of Gills Rot Fungus in Carp Fish. Journal of Applied Veterinary Sciences, 9(4), 54-59. doi: 10.21608/javs.2024.301499.1373
Zahraa Mustafa Al-Jumaa; Shahbaa Khalil AL-Taee; Mohammed Tariq Jaber; Aseel Mohammed Rahhawi. "Histopathological Alteration and Molecular Detection of Gills Rot Fungus in Carp Fish". Journal of Applied Veterinary Sciences, 9, 4, 2024, 54-59. doi: 10.21608/javs.2024.301499.1373
Al-Jumaa, Z., AL-Taee, S., Jaber, M., Rahhawi, A. (2024). 'Histopathological Alteration and Molecular Detection of Gills Rot Fungus in Carp Fish', Journal of Applied Veterinary Sciences, 9(4), pp. 54-59. doi: 10.21608/javs.2024.301499.1373
Al-Jumaa, Z., AL-Taee, S., Jaber, M., Rahhawi, A. Histopathological Alteration and Molecular Detection of Gills Rot Fungus in Carp Fish. Journal of Applied Veterinary Sciences, 2024; 9(4): 54-59. doi: 10.21608/javs.2024.301499.1373
Histopathological Alteration and Molecular Detection of Gills Rot Fungus in Carp Fish
1Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
2Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
3Forensic DNA Center for Research and Training, Al-Nahrain University, Baghdad, Iraq
Receive Date: 10 July 2024,
Revise Date: 22 August 2024,
Accept Date: 26 August 2024
Abstract
Nowadays, diseases are a major concern in fish production, particularly in gills, which play a crucial role in blood oxygenation, acid-base balance, osmoregulation, and waste elimination. Fungal infections, particularly Branchiomyces, cause respiratory problems in fish. This study aims to determine the causative agent causing high mortality in common carp, Cyprinus carpio, in the middle Euphrates in Iraq. One hundred infected fish were sampled for microbial, histological, and molecular examination. Gill tissue was examined as well for histological alterations, and DNA was isolated from Branchiomyces and amplified with universal primers. Microscopically identifying Branchiomyces spp. using lactophenol staining, non-separated hyphae and spores (5–9 m in size) at various stages inside the structures of gill tissue were present. Histopathological examination revealed hyperplasia of epithelial cells and infusion of gill filaments, while primary gill filaments displayed severe hemorrhage and edema. Hyphae of Branchiomyces have been detected between necrotic and edematous myofiber. The ITS PCR products of the fungal isolate were found to be positive at 540 bp molecular weight. Economic losses can occur as a result of fish infections caused by Branchiomyces. Histopathological lesions in gill tissue indicated the presence of Branchiomyces infection, which was confirmed by molecular and microscopical examination.
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